Hnology for their helpful remarks. We now have revised our manuscript accordingly. > 자유게시판

Hnology for his or her helpful comments. Now we have revised our manuscript appropriately.Reviewer #1: Professor Lutz WalterZimin and co-workers re-sequenced the Indian rhesus macaque person that was utilized PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/21046519 for that 1st genome assembly (rheMac2). They combined their Illumina sequences (entire genome as well as exome sequencing) with the primary Sanger sequences (of rheMac2) to make a whole new rhesus macaque genome assembly which they termed MacaM. These efforts resulted within a weighted average contig measurement remaining two times as massive given that the unique rheMac2 assembly and five moments much larger compared to CR_1.0 (Chinese rhesus macaque) assembly as well as in correction of various misassemblies. Inclusion of Illumina RNA-seq information from various tissues (cerebral cortex, caudate nucleus, thymus, testis) improved PRIMA-1 gene annotations. Further, the authors employed RNA-seq information from peripheral blood mononuclear cells (PBMCs) to show the remarkable precision and ability with the MacaM assembly to align reads from RNA-seq experiments. The former rhesus macaque genome assemblies and gene annotations urgently desired an update, which Robert Norgren's team delivered with the appropriate time. Their knowledge will help scientists to use the rhesus macaque genome far more efficiently. Authors' response: We thank Professor Walter for his type analysis. Distinct remarks:Zimin et al. Biology Immediate 2014, nine:20 http://www.biologydirect.com/content/9/1/Page twelve of4) Statistical analyses in the details demonstrated in Figures 4C and 5 are missing. Authors' response: We didn't supply a statistical comparison with the expression evaluation of the different behavioral groups for the reason that a manuscript focused on this issue is in preparation. To the present-day do the job, our emphasis was within the dissimilarities amongst the 2 assemblies. Figures 4C and five were presented to indicate how widespread these differences are. Considering that Dr. Yi (reviewer 2) experienced a related worry, we've deleted the last paragraph during the Final results segment associated with the various behavioral teams. five) I especially admit the new rhesus macaque chromosome nomenclature presented in Desk one, as there are already baffling reviews in past times and nomenclatures of chromosomes have been posted that obviously contradicted revealed info. Authors' response: We value Professor Walter's comment on this concern.Reviewer #2: Dr. Soojin YiIn this manuscript the authors deliver a brand new PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/3021955 sequencing and assembly of a rhesus macaque applying the animal utilized to create the commonly utilized RheMac1 assembly. Rhesus macaque is really a promising nonhuman primate model species and the utility of the enhanced rhesus macaque genome assembly for biomedical and comparative research is remarkable. This paper has plentiful deserves and i seem forward to employing the new assembly for investigate and training while in the around long run. The obvious merits of the function involve 1) combining Sanger and Illumina reads for assembly 2) validation of assembly with Ion Torrent reads 3) immediate comparison of the efficiencies of different assemblies for differential gene expression studies. The RNA sequencing and transcript assembly method as well as annotation system are fantastic. Authors' reaction: We thank Dr. Yi for her generous evaluation of our do the job. I've a number of specialized feedback the majority of that are for clarifications or particulars which will be valuable for long term people. p.2 and through the manuscript: the authors consult with the rheMac3 assembly as CR_1.0. It could help steering clear of confusion when the authors utilised a far more extensively.